Metformin is a well-known anti-diabetic drug. The current technology offers the opportunity to use Metformin, as second medical use, for the treatment of multiple sclerosis and graft-versus-host disease.

DKFZ together with Technical University of Denmark (DTU) developed an analogue of Griseofulvin as a compound for cancer treatment, which selectively targets cancer cells only.

L1 is overexpressed in highly malignant carcinomas and human ovarian tumors. The antibody 9.3 has improved anti-tumor capacities by inhibit tumor growth and invasion of tumor cells. Antibody can be also used to determine the L1 level in a patient.

“Ready to use kit” for simultaneous quantitative detection of up to 51 HPV genotypes. Kit contains internal quality and performance controls and allows identification of cervical abnormalities by predefined high viral load cutoffs.

Quadscanner for High Resolution Scanning Microscopes and Array Readers. The key facts are: (1) Fast, adaptive optical scanning device; (2) Completely free positioning of the scanning mirrors regarding the conjugated back focal plane; (3) Calibration with 4 galvanometers; (4) Application in high resolution microscopes (fluorescence, Raman, STED, localization), but also in Array Readers.

The technology specifies a novel device which allows to stabilize images of optical microscopes with high resolutions against externally induced oscillations and drift effects. In particular for optical microscopes, which achieve a higher resolution, it is important to ensure a high mechanical stability of the structure of microscope for a good image quality. It improves the performance of instruments such as laser scanners, fluorescence microscopes, Raman systems, STED- or localization-microscopes.

Optical techniques, such as bioluminescence and fluorescence, are emerging as powerful new modalities for molecular imaging in disease and therapy. Combining innovative molecular biology and chemistry, researchers have developed optical methods for imaging a variety of cellular and molecular processes in vivo, including protein interactions, protein degradation, and protease activity. DKFZ developed an optical imaging detector regarding fluorescence and bioluminescence for small animal imaging, which is compatible for magnetic resonance imaging (MRI). This technology provides the possibility to study simultaneously tracer/marker kinetics of optical (OT) as well as NMR induced signals.

DKFZ developed an optical imaging detector (25 mm x 100 mm photon sensor) for fluorescence and bioluminescence in small animal imaging, which is compatible for magnetic resonance imaging (MRI), computer tomography (CT), positron electron tomography (PET) and Single-photon emission computed tomography (SPECT). The instrument has been evaluated regarding ist optical performance including radiation durability using various phantoms and measurement setups and was successfully used in a number of preclinical studies such as simultaneous positron (18F-FDG, 68Ga-RGD) – bioluminescence (PC-3-hVEGF-Luc) imaging of reporter gene expression and receptor targeting in mice or simultaneous imaging of fluorescent XenoLight-RediJect-2-DG-750 and radiolabeled FDG probes.

The invention comprises a dermal/epidermal skin equivalent for studying epidermal development, the role of stem cells in human skin as well as the response to exogenous stimuli such as compounds used in cosmetic or pharmaceutical mixtures.

The site-directed modification of e.g. biomolecules is often time consuming and laborious. This invention provides a high yielding and highly specific method to link nearly any molecule to nearly any other molecule. The “Click reaction” used is the well known Diels-Alder reaction with inverse electron demand. In this fast reaction an appropriate, easily functionalized dien (e.g. Tetrazin) is reacted with an appropriate functionalized dienophil (e.g. Olefin) to yield the conjugate. The release of nitrogen makes the reaction irreversible. No side reactions occur. Further the Intellectual Property covers specific information regarding the ligation of a diagnostic marker AND a therapeutic agent AND a biomolecule. Convincing synthetic data are available for the ligation of peptide with peptide, peptide with marker molecule, peptide with therapeutic agent, peptide with oligo nucleotide, dendritic structure and specific surface modification by means of this technology.

High speed imaging in single spot laser scanning imaging is limited by saturation effects of the fluorescence process in the sample as well as by destructive processes due to the high intensity in the focal spot. The only way to overcome that limitation is to use multiple spots at the same time. We developed a Multi-Photon Multi-Spot Scanning Device which is applicable especially in laser scanning devices such as confocal laser scanners and multi-photon microscopes. It can be used to speed up imaging of living cells which would be harmed by a stronger single beam.

DKFZ developed an optical imaging detector for fluorescence and bioluminescence in small animal imaging, which is compatible for positron electron tomography (PET). Compatibility of light detection with PET has been accomplished by developing an optical detector that consists of a 25 mm x 100 mm photon sensor (liquid cooled) for light detection, a microlens array for field-of-view definition, a septum masks for cross-talk suppression, and a transferable filter for wavelength selection.

This invention describes a simple and robust navigation aid for physicians in bronchoscopy or similar interventional surgery (angiography; implanting of vessel stents as well as cardiac pacemaker). The interventional device can be monitored and in real time and at a defined localized spot a biopsy can be taken. It improves accuracy also in the case of movement of the organ structures in opposite to established procedures. A prototype phantom has been tested successfully at the DKFZ together with the software.

Optical fluorescence and gamma-labeled radio-pharmaceutical imaging offer complementary ways of probing and assessing the physiology of small animals. With the increasing use of both methods for functional molecular imaging in small animal models for applications such as cancer research and drug development, it has become more important to integrate their results, particularly for studies of the same animal. This can be performed through image registration of separate studies or through simultaneous tomographic acquisition with implicit registration of the two imaging modalities. DKFZ developed an optical imaging detector for fluorescence and bioluminescence in small animal imaging, which is compatible for single-photon emission computed tomography (SPECT).

New method for producing a highly diverse antibody library, where pro-teins/antibodies can be selected for specific applications. A large and, compared from cell to cell, highly uniform number of antibodies is covalently attached to the surface of a eukaryotic cell. Using this technology, specific antibodies can be selected together with the presenting cell out of a high number of cells in a comparatively easy way. The technology can also be used humanizing a hybridoma cell line by enabling the homologous recombination of the constant do-mains of human immunoglobulins with the respective gene.

The present technology provides 3'-photolabile nucleoside derivatives for producing nucleotides. The resulting oligonucleotides built up on the support by light-controlled synthesis are linked to the solid phase via the 5' end and thus can be used for investigating reactions that require free 3'-OH ends, for example certain DNA-enzyme interactions.