TAPPIR® - Tunable Aqueous Polymer Phase Impregnated Resins
Aqueous Two-Phase Extraction (ATPE) is a process proved efficient for the gentle purification of various biomolecules. Fast mass transfer rates and less damage to proteins that are sensitive to mechanical stresses and organic solvents render this unit operation an alternative to commonly used downstream processing technique.
However ATPE has no large-scale industrial applications yet. This is based substantially on very long phase separation times resulting from low interfacial tensions and low density differences between the phases producing intensive and stable emulsions. This causes an increased footprint of the required mixer/settler devices or the application of additional equipment such as energy consuming centrifuges.
The present invention relates to a process for separation or purification of biomolecules via ATPE. The advantages are maintained and the major limitation of long phase separation time is overcome by immobilizing one of the two phases in porous particles. Suspending the impregnated particles in the second aqueous phase containing the biomolecules creates the emulsion without any need for phase separation after the extraction step.
During the separation process, the biomolecules partition between the aqueous phase surrounding the particles and the immobilized second aqueous phase inside of the particles’ pores.
The TAPPIR®-technology can be used on one hand for separation which means the isolation of biomolecules from e.g. fermentation broth (Capture), on the other hand for purification which means the removal of biomolecules like objectionable proteins, viruses, DNA, lipids or other cell debris from biocatalytic processes within the separation of metabolites (Clarification). Moreover the flexibility of the TAPPIR®-technology extends to different possible operation modes namely packed bed, fluidized bed or in situ for e.g. extractive fermentation.
In a first example, BSA partitioning in a PEG/Citrate-TAPPIR®-system was characterized. The depletion of BSA in the aqueous salt phase could be increased by 10% in relation to conventional extraction methods. This means nearly a twofold higher efficiency by the use of a considerably faster separation process.
Weitere Informationen: PDF
Tel.: +49 (0)208/94105 10
Dipl.-Ing. Alfred Schillert
firstname.lastname@example.org | TechnologieAllianz e.V.
Die aktuellsten Pressemeldungen zum Suchbegriff Innovation >>>
Die letzten 5 Focus-News des innovations-reports im Überblick:
Forscher der Universität Göttingen entdecken zusätzliche Funktion von Opsinen
Die Fotorezeptoren in den Augen von Tieren fangen Licht mithilfe von Fotopigmenten ein. Forscher der Universität Göttingen haben nun entdeckt, dass diese...
Researchers from Göttingen University discover additional function of opsins
Animal photoreceptors capture light with photopigments. Researchers from the University of Göttingen have now discovered that these photopigments fulfill an...
Röntgenholographie im Flug - Eine neue Methode der Röntgenholographie erzeugt Bilder von Partikeln in der Gasphase
Wissenschaftlerinnen und Wissenschaftler in der Arbeitsgruppe von Prof. Thomas Möller am Institut für Optik und Atomare Physik der TU Berlin ist es gemeinsam...
Researchers embark on aerial campaign over Northeast Greenland
On 15 March, the AWI research aeroplane Polar 5 will depart for Greenland. Concentrating on the furthest northeast region of the island, an international team...
Data collected on ocean-ice interactions in the little-researched regions of the far south
The world’s second-largest ice shelf was the destination for a Polarstern expedition that ended in Punta Arenas, Chile on 14th March 2018. Oceanographers from...