Immunization – ImmunEasy™ Mouse Adjuvant

For induction of high antibody titers in mice

Features and benefits

• Easy mixing and delivery in aqueous suspension
• Rapid production of high antibody titers
• Boosting performed after only 2 weeks
• Less antigen required than with conventional methods
• Non-toxic adjuvant with no adverse side effects

Principle

ImmunEasy™ Mouse Adjuvant is a new type of adjuvant specifically designed for inducing high antibody titers in mice. The adjuvant contains immunostimulatory CpG™ DNA (1–4) — short oligonucleotides that contain unmethlylated cytosine-guanine dinucleotides within a certain base context. The mammalian immune system has evolved to recognize these sequences, which are found naturally in bacterial DNA, as a sign of infection. Exposure to CpG DNA results in a very rapid and strong immune response, and, when applied with an antigen, CpG DNA produces high titers of antigen-specific antibodies. The antibody titer induced by ImmunEasy Mouse Adjuvant is generally equal to or higher than that induced by Freund’s complete adjuvant. In addition, high antibody titers are induced using up to 10-fold less antigen compared to some other adjuvants. Furthermore, boosting can be performed after 2 weeks instead of 4, saving both time and animal-housing costs.

Procedure

ImmunEasy Mouse Adjuvant is provided as a ready-to-use aqueous suspension. The procedure is easy — adjuvant and antibody are mixed, incubated for 5–10 min at room temperature, and then injected into the mouse. Tedious mixing procedures such as heating, sonification, lyophilization, and homogenization, which are required for Freund’s adjuvant and other water-in-oil emulsions, are completely avoided, as are problems associated with improperly prepared adjuvant-antigen mixes. Boosting is performed 2 weeks after the primary immunization

More Information