Forum für Wissenschaft, Industrie und Wirtschaft

Hauptsponsoren:     3M 
Datenbankrecherche:

 

Growth factor aids stem cell regeneration after radiation damage

04.02.2013
Epidermal growth factor has been found to speed the recovery of blood-making stem cells after exposure to radiation, according to Duke Medicine researchers. The finding could open new options for treating cancer patients and victims of dirty bombs or nuclear disasters.

Reported in the Feb. 3, 2013, issue of the journal Nature Medicine, the researchers explored what had first appeared to be an anomaly among certain genetically modified mice with an abundance of epidermal growth factor in their bone marrow. The mice were protected from radiation damage, and the researchers questioned how this occurred.

"Epidermal growth factor was not known to stimulate hematopoiesis, which is the formation of blood components derived from hematopoietic stem cells," said senior author John Chute, M.D., a professor of medicine and professor of pharmacology and cancer biology at Duke University. "However, our studies demonstrate that the epidermal growth promotes hematopoietic stem cell growth and regeneration after injury."

Hematopoietic stem cells, which constantly churn out new blood and immune cells, are highly sensitive to radiation damage. Protecting these cells or improving their regeneration after injury could benefit patients who are undergoing bone marrow transplantation, plus others who suffer radiation injury from accidental environmental exposures such as the Japanese nuclear disaster in 2011.

The Duke researchers launched their investigation using mice specially bred with deletions of two genes that regulate the death of endothelial cells, which line the inner surface of blood vessels and are thought to regulate the fate of hematopoietic stem cells. Blood vessels and the hematopoietic system in these mice were less damaged when exposed to high doses of radiation, improving their survival.

An analysis of secretions from bone marrow endothelial cells of the protected mice showed that epidermal growth factor (EGF) was significantly elevated - up to 18-fold higher than what was found in the serum of control mice. The researchers then tested whether EGF could directly spur the growth of stem cells in irradiated bone marrow cultured in the lab. It did, with significant recovery of stem cells capable of repopulating transplanted mice.

Next, the Duke team tried the approach in mice using three different solutions of cells in animals undergoing bone marrow transplants. One group received regular bone marrow cells; a second group got bone marrow cells from donors that had been irradiated and treated with EGF; a third group got bone marrow cells from irradiated donors treated with saline.

The regular bone marrow cells proliferated well and had the highest rate of engraftment in the recipient mice. But mice that were transplanted with the cells from irradiated/EGF-treated donors had 20-fold higher engraftment rate than the third group.

Additional studies showed that EGF improved survival from a lethal radiation exposure, with 93 percent of mice surviving the radiation dose if they subsequently received treatment with EGF, compared to 53 percent surviving after treatment with a saline solution.

Chute said it appears that EGF works by repressing a protein called PUMA that normally triggers stem cell death following radiation exposure.

"We are just beginning to understand the mechanisms through which EGF promotes stem cell regeneration after radiation injury," Chute said. "This study suggests that EGF might have potential to accelerate the recovery of the blood system in patients treated with chemotherapy or radiation."

In addition to Chute, study authors include Phuong L. Doan, Heather A. Himburg, Katherine Helms, J. Lauren Russell, Emma Fixsen, Mamle Quarmyne, Jeffrey R. Harris, Divino Deoliviera, Julie M. Sullivan, Nelson J. Chao and David G. Kirsch.

The study was funded by the National Heart, Lung and Blood Institute (HL-086998-01); the National Institute of Allergy and Infectious Diseases (AI-067798-06, AI-067798-01); the National Institutes of Health (T32 HL0070757-33); the Barton Haynes Award and Duke University.

Sarah Avery | EurekAlert!
Further information:
http://www.duke.edu

More articles from Health and Medicine:

nachricht Mass. General team generates therapeutic nitric oxide from air with an electric spark
07.07.2015 | Massachusetts General Hospital

nachricht UNC researchers find 2 biomarkers linked to severe heart disease
07.07.2015 | University of North Carolina Health Care

All articles from Health and Medicine >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Molekulare Container für die Gewinnung Seltener Erden: Neues Forschungsprojekt „SE-FLECX“ gestartet

Seltene Erden sind begehrt, weil sie für die Herstellung vieler Hightech-Produkte benötigt werden. Allerdings ist ihre Gewinnung aus Erzen aufwendig und häufig umweltbelastend.

Experten aus Forschung und Industrie testen derzeit unter Koordination des Helmholtz-Instituts Freiberg für Ressourcentechnologie, ob so genannte Calixarene...

Im Focus: Surfing a wake of light

Researchers observe and control light wakes for the first time

When a duck paddles across a pond or a supersonic plane flies through the sky, it leaves a wake in its path. Wakes occur whenever something is traveling...

Im Focus: Auf den Quantenpunkt gebracht: ultrahochauflösendes 3D-Mikroskopieverfahren für elektrische Felder

Mit einem einzelnen Molekül als Sensor ist es Jülicher Wissenschaftlern gelungen, elektrische Potenzialfelder in bisher unerreichter Präzision abzubilden. Die ultrahochaufgelösten Aufnahmen geben Aufschluss über die Verteilung von Ladungen in der Elektronenhülle einzelner Moleküle und sogar Atome. Die Methode arbeitet berührungsfrei und in 3D.

Erste Ergebnisse der als Raster-Quantenpunkt-Mikroskopie bezeichneten Technik sind in der aktuellen Ausgabe der Fachzeitschrift Physical Review Letters...

Im Focus: Magnettests an Wendelstein 7-X erfolgreich abgeschlossen

Wichtiger Schritt der Betriebsvorbereitung / erstes Plasma in der Fusionsanlage rückt näher

Mit dem erfolgreich abgeschlossenen Test aller siebzig Magnetspulen ist jetzt die Funktion der technologischen Schlüsselkomponente für das Fusionsexperiment...

Im Focus: Neues Verfahren ermöglicht 3D-Auslesung von magnetischen Mustern

Eine internationale Kooperation hat es geschafft, mit Synchrotronlicht komplexe Magnetisierungsmuster in gewickelten magnetischen Schichten dreidimensional auszulesen. Dieses Verfahren könnte interessant sein, um hochempfindliche Sensoren für Magnetfelder zu entwickeln, beispielsweise für die medizinische Diagnostik. Die Ergebnisse sind in Nature Communications publiziert.

Feine Strukturen im Inneren von Materialien und biologischen Proben lassen sich heute mit Röntgentomographie gut ermitteln: Dabei werden Schicht für Schicht...

Alle Focus-News des Innovations-reports >>>

Anzeige

Anzeige

IHR
JOB & KARRIERE
SERVICE
im innovations-report
in Kooperation mit academics
Veranstaltungen

Global Communication Association Konferenz: Drei Tage im Zeichen des digitalen Wandels

07.07.2015 | Veranstaltungen

IMTC 2015: Internationale Fachtagung im Kompetenzfeld Leichtbau

06.07.2015 | Veranstaltungen

Rheumatologen tagen in Bremen: Fortschritte in der Rheuma-Therapie und neue Impfempfehlungen

06.07.2015 | Veranstaltungen

 
B2B-VideoLinks
Weitere VideoLinks >>>
Aktuelle Beiträge

Ein schwarzes Loch unter der Gravitationslinse

07.07.2015 | Physik Astronomie

Nanodraht-Netz sagt Muskelkater den Kampf an

07.07.2015 | Medizin Gesundheit

Studie über Bio-Hacking: Öffnen wir unsere Haustüren bald mit Chip-Implantaten?

07.07.2015 | Medizin Gesundheit