Forum für Wissenschaft, Industrie und Wirtschaft

Hauptsponsoren:     3M 
Datenbankrecherche:

 

Growth factor aids stem cell regeneration after radiation damage

04.02.2013
Epidermal growth factor has been found to speed the recovery of blood-making stem cells after exposure to radiation, according to Duke Medicine researchers. The finding could open new options for treating cancer patients and victims of dirty bombs or nuclear disasters.

Reported in the Feb. 3, 2013, issue of the journal Nature Medicine, the researchers explored what had first appeared to be an anomaly among certain genetically modified mice with an abundance of epidermal growth factor in their bone marrow. The mice were protected from radiation damage, and the researchers questioned how this occurred.

"Epidermal growth factor was not known to stimulate hematopoiesis, which is the formation of blood components derived from hematopoietic stem cells," said senior author John Chute, M.D., a professor of medicine and professor of pharmacology and cancer biology at Duke University. "However, our studies demonstrate that the epidermal growth promotes hematopoietic stem cell growth and regeneration after injury."

Hematopoietic stem cells, which constantly churn out new blood and immune cells, are highly sensitive to radiation damage. Protecting these cells or improving their regeneration after injury could benefit patients who are undergoing bone marrow transplantation, plus others who suffer radiation injury from accidental environmental exposures such as the Japanese nuclear disaster in 2011.

The Duke researchers launched their investigation using mice specially bred with deletions of two genes that regulate the death of endothelial cells, which line the inner surface of blood vessels and are thought to regulate the fate of hematopoietic stem cells. Blood vessels and the hematopoietic system in these mice were less damaged when exposed to high doses of radiation, improving their survival.

An analysis of secretions from bone marrow endothelial cells of the protected mice showed that epidermal growth factor (EGF) was significantly elevated - up to 18-fold higher than what was found in the serum of control mice. The researchers then tested whether EGF could directly spur the growth of stem cells in irradiated bone marrow cultured in the lab. It did, with significant recovery of stem cells capable of repopulating transplanted mice.

Next, the Duke team tried the approach in mice using three different solutions of cells in animals undergoing bone marrow transplants. One group received regular bone marrow cells; a second group got bone marrow cells from donors that had been irradiated and treated with EGF; a third group got bone marrow cells from irradiated donors treated with saline.

The regular bone marrow cells proliferated well and had the highest rate of engraftment in the recipient mice. But mice that were transplanted with the cells from irradiated/EGF-treated donors had 20-fold higher engraftment rate than the third group.

Additional studies showed that EGF improved survival from a lethal radiation exposure, with 93 percent of mice surviving the radiation dose if they subsequently received treatment with EGF, compared to 53 percent surviving after treatment with a saline solution.

Chute said it appears that EGF works by repressing a protein called PUMA that normally triggers stem cell death following radiation exposure.

"We are just beginning to understand the mechanisms through which EGF promotes stem cell regeneration after radiation injury," Chute said. "This study suggests that EGF might have potential to accelerate the recovery of the blood system in patients treated with chemotherapy or radiation."

In addition to Chute, study authors include Phuong L. Doan, Heather A. Himburg, Katherine Helms, J. Lauren Russell, Emma Fixsen, Mamle Quarmyne, Jeffrey R. Harris, Divino Deoliviera, Julie M. Sullivan, Nelson J. Chao and David G. Kirsch.

The study was funded by the National Heart, Lung and Blood Institute (HL-086998-01); the National Institute of Allergy and Infectious Diseases (AI-067798-06, AI-067798-01); the National Institutes of Health (T32 HL0070757-33); the Barton Haynes Award and Duke University.

Sarah Avery | EurekAlert!
Further information:
http://www.duke.edu

More articles from Health and Medicine:

nachricht Punctuating messages encoded in human genome with transposable elements
04.08.2015 | Aelan Cell Technologies

nachricht Real-time imaging of lung lesions during surgery helps localize tumors and improve precision
30.07.2015 | American Association for Thoracic Surgery

All articles from Health and Medicine >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Kleine Löcher, große Wirkung: Aktives Elastomerlager reduziert Schwingungen

Wo große Bewegungen ausgeglichen werden müssen, sind Elastomere in ihrem Element. Sie federn passiv Stöße bei Fahrzeugen ab und reduzieren Schwingungen in Maschinen. Aber sie können noch mehr als das, wie Forscher des Fraunhofer LBF zeigen konnten. Sie haben diese elastischen Komponenten smarter gemacht und ihnen beigebracht, sich aktiv zu verformen. Dazu nutzt das Institut dielektrische Elastomere (DE). Das sind weiche Materialien, die sich unter hohen elektrischen Spannungen verformen, und das prädestiniert sie für den Aufbau von Aktoren. Gegenüber Piezoaktoren haben sie den Vorteil, vergleichsweise große Dehnungen bei geringeren Kräften zu erreichen.

Diese Fähigkeit haben die Wissenschaftler des Fraunhofer-Instituts für Betriebsfestigkeit und Systemzuverlässigkeit LBF genutzt und ein Konzept für DE-Aktoren...

Im Focus: Greenhouse gases' millennia-long ocean legacy

Continuing current carbon dioxide (CO2) emission trends throughout this century and beyond would leave a legacy of heat and acidity in the deep ocean. These...

Im Focus: Kosten sparen beim Bau von Flugzeugturbinen

Verdichterscheiben für Flugzeugturbinen werden aus einem Materialstück herausgefräst. Bei der Bearbeitung fangen die Schaufeln an zu schwingen. Ein neuartiges Spannsystem steigert die Dämpfung der Schaufeln nun auf mehr als das 400-fache. Es lassen sich bis zu 5000 Euro Kosten bei der Fertigung einsparen.

Mal eben schnell in den Urlaub jetten oder für ein langes Wochenende nach Rom, Paris oder Madrid fliegen? Der Flugverkehr steigt, insbesondere der...

Im Focus: Gletscher verlieren mehr Eis als je zuvor

Der Gletscherschwund im ersten Jahrzehnt des 21. Jahrhunderts erreicht einen historischen Rekordwert seit Messbeginn. Das Schmelzen der Gletscher ist ein globales Phänomen und selbst ohne weiteren Klimawandel werden sie zusätzlich an Eis verlieren. Dies belegt die neueste Studie des World Glacier Monitoring Services unter der Leitung der Universität Zürich.

Seit über 120 Jahren sammelt der World Glacier Monitoring Service, mit heutigem Sitz an der Universität Zürich, weltweite Daten zu Gletscherveränderungen....

Im Focus: Glaciers melt faster than ever

Glacier decline in the first decade of the 21st century has reached a historical record, since the onset of direct observations. Glacier melt is a global phenomenon and will continue even without further climate change. This is shown in the latest study by the World Glacier Monitoring Service under the lead of the University of Zurich, Switzerland.

The World Glacier Monitoring Service, domiciled at the University of Zurich, has compiled worldwide data on glacier changes for more than 120 years. Together...

Alle Focus-News des Innovations-reports >>>

Anzeige

Anzeige

IHR
JOB & KARRIERE
SERVICE
im innovations-report
in Kooperation mit academics
Veranstaltungen

Managementkonferenz

04.08.2015 | Veranstaltungen

Tagung "Intelligente Beschichtungen für Außenanwendungen" in Dresden

03.08.2015 | Veranstaltungen

MS Wissenschaft in Stuttgart: Fraunhofer zeigt Chancen im Ländle auf

03.08.2015 | Veranstaltungen

 
B2B-VideoLinks
Weitere VideoLinks >>>
Aktuelle Beiträge

Siemens modernisiert Großteil des belgischen Eisenbahnnetzes

04.08.2015 | Verkehr Logistik

Wegweisende Konzepte rund ums Automatisierte Fahren und innovative Fahrzeuge gesucht

04.08.2015 | Verkehr Logistik

Mit Hightech und Honigtöpfen gegen Hacker

04.08.2015 | Informationstechnologie