Forum für Wissenschaft, Industrie und Wirtschaft

Home Fachgebiete Biowissenschaften Chemie Nachricht

Unusually Large Family of Green Fluorescent Proteins Discovered in Marine Creature

nächste Meldung

Researchers at Scripps Institution of Oceanography at UC San Diego and the Salk Institute for Biological Studies have discovered a family of green fluorescent proteins (GFPs) in a primitive sea animal, along with new clues about the role of the proteins that has nothing to do with their famous glow.

GFPs recently gained international attention with the awarding of the 2008 Nobel Prize in Chemistry, shared by UC San Diego’s Roger Tsien, as word spread of their extensive presence in nature as well as benefit to researchers. GFPs, originally isolated from a luminous jellyfish, have gained scientific ubiquity in uses ranging from biomedical tracers to probes for testing environmental quality. But while the value of GFPs in biomedicine and bioengineering has become evident, their diversity across the tree of life and their role in nature haven’t been as easily deciphered.

New hints have emerged as Erin Bomati, a former postdoctoral researcher at Scripps Oceanography, Gerard Manning of the Salk Institute for Biological Studies and Scripps lead-scientist Dimitri Deheyn discovered the largest known family of GFPs. They found 16 related types of GFPs in amphioxus, a thin, non-luminous fish-like animal that lives in coastal areas and spends most of its time burrowed in ocean sand. The discovery, described in the journal BioMed Central (BMC) Evolutionary Biology, was made in Branchiostoma floridae, an amphioxus species collected off Tampa, Fla.

Amphioxus, also known as lancelets, is the closest living invertebrate relative of vertebrates and much more evolved than the jellyfish in which the original GFP discovery was made. In the paper, the researchers demonstrate that the 16 newly discovered GFPs have different characteristics of light production, some brightly fluorescent and others less or not at all.

“Despite a huge knowledge base about the biochemistry of GFPs, little is know about their biological functions and our results clearly indicate that it is not always related to fluorescence,” said Deheyn.

Using a range of genetic analyses and techniques, including sequencing and cloning, the researchers discovered that some GFPs, especially those with low fluorescence capacity, could have a defense function in the wild acting as an antioxidant, working to protect the animal in times of illness or stress. It’s the first evidence of the proteins being used in a role beyond glowing fluorescence within the same organism.

“Originally GFPs might have been selected for their function of being able to absorb or re-emit light, but here we show that some GFPs can also act as antioxidants,” said Deheyn. “This is the first time that we have identified distinct functions in coexisting GFPs.”

Deheyn said GFPs appear to suppress so-called “oxygen radicals” from harmful effects to the amphioxus’ body, similar to the role antioxidants serve in human bodies.

Deheyn said the new findings will help scientists understand the evolution of this protein across the animal kingdom, while providing bioengineers and biotechnologists a new window of comparison through the novel family of GFPs and an unveiled aspect of their application. The range of colors and functions encoded by these GFPs may also help to decode which aspects of their sequences are responsible for which functions and the engineering of new forms of GFP probes.

The research was supported by the Air Force Office of Scientific Research’s Biomimetics, Biomaterials and Biointerfacial Sciences program.

Note to broadcast and cable producers: UC San Diego provides an on-campus satellite uplink facility for live or pre-recorded television interviews. Please phone or e-mail the media contact listed above to arrange an interview.

Scripps Institution of Oceanography: scripps.ucsd.edu

Scripps News: scrippsnews.ucsd.edu

Scripps Institution of Oceanography, at UC San Diego, is one of the oldest, largest and most important centers for global science research and education in the world. The National Research Council has ranked Scripps first in faculty quality among oceanography programs nationwide. Now in its second century of discovery, the scientific scope of the institution has grown to include biological, physical, chemical, geological, geophysical and atmospheric studies of the earth as a system. Hundreds of research programs covering a wide range of scientific areas are under way today in 65 countries. The institution has a staff of about 1,300, and annual expenditures of approximately $155 million from federal, state and private sources. Scripps operates one of the largest U.S. academic fleets with four oceanographic research ships and one research platform for worldwide exploration.

Mario Aguilera | Quelle: Newswise Science News
Weitere Informationen: www.ucsd.edu

nächste Meldung