Forum für Wissenschaft, Industrie und Wirtschaft

Home Fachgebiete Biowissenschaften Chemie Nachricht

SPR Biosensor systems

nächste Meldung

XanTec’s SPR biosensor program is currently undergoing some actualization. Below you will find general information about our SPR technology. In this section you will also find the details of the new systems as soon as they are available. If you are interested in information on the IBIS^® series, which we do not offer anymore, please click here.

SPR: A powerful tool for real-time analysis of biomolecular interactions

The study and characterization of molecular interactions is essential to explore the structure-function relationships of biomolecules, aiding to our understanding of biological systems in life sciences. Surface Plasmon Resonance biosensors analyze macromolecular interactions in real-time and label-free. They thus provide scientists with a valuable tool for studies in the fields of immunology, molecular biology, cell biology and biochemistry. Using SPR biosensors, cell adhesion studies, quality control of immunoreagents, drug development and investigation of polymer- biomolecule interactions for biomaterial are speeded up several times compared to conventional techniques.

Listed below are some examples of biomolecular interactions which have been successfully studied using SPR:

• Peptide/protein - protein

• DNA/RNA - protein

• protein - cell

• receptor - cell

• protein - virus/phage

• carbohydrate - protein

• carbohydrate - cell

• liposome - protein

• artificial materials - biological matter

• drugs - protein

• drugs - DNA/RNA.

In addition to biomolecular interaction studies, SPR sensors can be used to quantify adsorption and desorption processes in non-biological systems. One of these less known applications is the quick evaluation of new detergent formulations by real time analysis of dirt desorption from SPR sensor surfaces.

Sensitive optical detection

Our optical biosensors, which use Surface Plasmon Resonance (SPR), measure refractive index changes close to the interface of a sensor chip in real-time. Such changes occur when biomolecules interact with immobilized ligands and replace water from binding sites on the sensor surface. The refractive index differences are detected via angle changes of a reflected laser beam and are directly related to the amount of sensor surface-bound biomolecules. Using this sensitive technique, our SPR sensors are able to detect protein amounts below 10 pg / mm2. For more details on SPR please check the chapter "SPR principle" in our Tech Note section

Liquid handling systems

Liquid handling systems control the hydrodynamic conditions on the sensor surface, which is essential for the performance of a biosensor system. Generally, today’s liquid handling systems can be divided into two groups: flow through systems and cuvette systems. In flow through systems, fresh sample is continually flown over the sensor surface. This method has the advantage of well-defined hydrodynamic conditions during a measurement without rebinding during the dissociation phase. However, the continuous sample consumption limits the maximal association time and, once the sample has been injected, it is difficult to abort the measurement or to inject additional compounds. In cuvette systems, the sample is injected into a well on the sensor surface and is continually mixed during the measurement. This principle is flexible enough to allow multiple sample additions, and it is relatively easy to interrupt the experiment at any time. Moreover, it is possible to measure binding processes with a relatively small sample volume over a very long time or until equilibrium. The latter is especially useful for analytes with a low association constant. For our SPR biosensors flow through and cuvette systems are available. The user thus has the possibility to choose for each experiment the optimal liquid handling system.

Wide variety of sensor surfaces

The sensor surface is the central part of a biosensor. It is here where the biomolecular interaction takes place and where the biochemical binding event is transduced into an opto-electronic signal. Therefore the quality of the sensor surface determines the quality of the interaction plots. As different chemical structures of the immobilized biomolecules require adapted coupling strategies, we offer the market-wide biggest choice of SPR sensordiscs derivatized with various chemical and biochemical functionalities. These functional groups are either linked to planar surfaces or attached to grafted hydrogels which are available in different thicknesses.

xantec | Quelle: xantec

nächste Meldung