Forum für Wissenschaft, Industrie und Wirtschaft

Hauptsponsoren:     3M 
Datenbankrecherche:

 

Tracking down smallest biomarkers

27.11.2012
PTB and Dectris have developed a vacuum-compatible X-ray detector that allows the size of low-contrast nano-objects to be determined.

Microvesicles are smallest cell elements which are present in all body fluids and are different, depending on whether a person is healthy or sick. This could contribute to detecting numerous diseases, such as, e.g., carcinomas, at an early stage, and to treating them more efficiently.


Small-angle X-ray scattering of a micro-vesicle sample (multilamellar liposomes) using the vacuum-compatible Pilatus detector, image recorded at a photon energy of 3 keV. The scattering pattern allows the dimensions of the nano-objects in the examined sample to be determined. (Fig.: PTB)

The problem is that the diameter of the relevant microvesicles generally lies below 100 nm, which makes them technically detectable, but their exact size and concentration hardly possible to determine. A new device is now to provide the metrological basis for these promising biomarkers.

The vacuum-compatible version of the Pilatus hybrid pixel detector for X-rays, which was developed by Dectris in cooperation with the Physikalisch-Technische Bundesanstalt (PTB), now allows also the size of nano-particles - which, to date, have been difficult to characterize - to be determined using small-angle X-ray scattering at low photon energies. The detector can also be used for other X-ray-based techniques.

What makes this detector unique is the size of its total surface (17 cm × 18 cm) as well as the fact that it can be operated in vacuum. Operating the detector in vacuum drastically increases the sensitivity of the measuring facility, since the soft X-rays, which are scattered on the sample, are not absorbed by air molecules on their way towards the detector.

This device now allows, for example, experiments for size determination of nanoparticles to be carried out with small-angle X-ray scattering (SAXS) also at the absorption edges of the light elements calcium, sulphur, phosphor or silicon at photon energies below 5 keV with high dynamics and good spatial resolution.

For a few months, the new Pilatus X-ray detector has been used for some of PTB's own research projects. At the synchrotron radiation source BESSY II in Berlin-Adlershof, where PTB has been operating its own laboratory for 15 years, scientists are now using the new detector, for example, to establish the - urgently needed - metrological basis for the size determination of microvesicles.

A project carried out within the scope of the European Metrology Research Programme (EMRP) and with the significant participation of the Amsterdam Medical Center in the Netherlands is to contribute decisively to fully exploiting the potential of microvesicles for the early diagnosis of diseases

For further information about the detector, please visit:
http://www.ptb.de/cms/nc/en/fachabteilungen/abt7/news-7/single-view.html?tx_ttnews[tt_news]=5214&tx_ttnews[backPid]=85&cHash=2b2e374f28a2b5eea11d24c8d9193736
https://www.dectris.com/successstories/items/In-vacuum-P1M-PTB_SuccStory.html#In-vacuum-P1M-PTB_SuccStory

For further information about the characterization of microvesicles, please visit:
http://www.euramet.org/fileadmin/docs/EMRP/JRP/JRP_Summaries_2011/Health_
JRPs/HLT02_Publishable_JRP_Summary.pdf

Contact:

Michael Krumrey,
PTB Working Group 7.11 "X-ray Radiometry",
phone: +49 (0)30 3481-7110,
e-mail: michael.krumrey@ptb.de
(untill 3. December contact is only possible by e-mail)

Michael Krumrey | EurekAlert!
Further information:
http://www.ptb.de

More articles from Life Sciences:

nachricht Subcutaneous Administration of Multispecific Antibody Makes Tumor Treatment Faster & More Tolerable
01.07.2015 | Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt

nachricht Why human egg cells don't age well
01.07.2015 | RIKEN

All articles from Life Sciences >>>

The most recent press releases about innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: Radar schützt vor Weltraummüll

Die Bedrohung im All durch Weltraummüll ist groß. Aktive Satelliten und Raumfahrzeuge können beschädigt oder zerstört werden. Ein neues, nationales Weltraumüberwachungssystem soll ab 2018 vor Gefahren im Orbit schützen. Fraunhofer-Forscher entwickeln das Radar im Auftrag des DLR Raumfahrtmanagement.

Die »Verkehrssituation« im All ist angespannt: Neben unzähligen Satelliten umkreisen Weltraumtrümmer wie beispielsweise ausgebrannte Raketenstufen und...

Im Focus: X-rays and electrons join forces to map catalytic reactions in real-time

New technique combines electron microscopy and synchrotron X-rays to track chemical reactions under real operating conditions

A new technique pioneered at the U.S. Department of Energy's Brookhaven National Laboratory reveals atomic-scale changes during catalytic reactions in real...

Im Focus: Neue Flugzeugflügel reparieren Risse von selbst

Prozess basiert auf Kohlefasern, die bei Schäden sofort aktiv werden

Forscher der University of Bristol http://bris.ac.uk haben Flugzeugflügel entwickelt, die sich im Falle eines Schadens selbst reparieren können. Als...

Im Focus: Aktuatoren – bewegt wie die Mittagsblume

Materialien nach dem Vorbild mancher Pflanzen könnten Robotern künftig zu natürlichen Bewegungen verhelfen

Wenn Ingenieure bewegliche Komponenten von Robotern entwickeln, können sie sich demnächst vielleicht der Kniffe von Pflanzen bedienen. Forscher des...

Im Focus: Iron: A biological element?

Think of an object made of iron: An I-beam, a car frame, a nail. Now imagine that half of the iron in that object owes its existence to bacteria living two and a half billion years ago.

Think of an object made of iron: An I-beam, a car frame, a nail. Now imagine that half of the iron in that object owes its existence to bacteria living two and...

Alle Focus-News des Innovations-reports >>>

Anzeige

Anzeige

IHR
JOB & KARRIERE
SERVICE
im innovations-report
in Kooperation mit academics
Veranstaltungen

Dauerbrenner Zusatzstoffe in Lebensmitteln

01.07.2015 | Veranstaltungen

Interdisziplinäres Forschungscluster „Demografischer Wandel“ tagte zum ersten Mal

01.07.2015 | Veranstaltungen

DBU lädt ein zu Forum über nachhaltige Landwirtschaft

01.07.2015 | Veranstaltungen

 
B2B-VideoLinks
Weitere VideoLinks >>>
Aktuelle Beiträge

Dauerbrenner Zusatzstoffe in Lebensmitteln

01.07.2015 | Veranstaltungsnachrichten

Neun neue Forschergruppen, eine neue Klinische Forschergruppe

01.07.2015 | Förderungen Preise

Offshore-Windkraftwerk Westermost Rough offiziell eingeweiht

01.07.2015 | Unternehmensmeldung