Forum für Wissenschaft, Industrie und Wirtschaft

Hauptsponsoren:     3M 
Datenbankrecherche:

 

SPR Biosensor systems

05.09.2001


XanTec’s SPR biosensor program is currently undergoing some actualization. Below you will find general information about our SPR technology. In this section you will also find the details of the new systems as soon as they are available. If you are interested in information on the IBIS® series, which we do not offer anymore, please click here.


SPR: A powerful tool for real-time analysis of biomolecular interactions

The study and characterization of molecular interactions is essential to explore the structure-function relationships of biomolecules, aiding to our understanding of biological systems in life sciences. Surface Plasmon Resonance biosensors analyze macromolecular interactions in real-time and label-free. They thus provide scientists with a valuable tool for studies in the fields of immunology, molecular biology, cell biology and biochemistry. Using SPR biosensors, cell adhesion studies, quality control of immunoreagents, drug development and investigation of polymer- biomolecule interactions for biomaterial are speeded up several times compared to conventional techniques.

Listed below are some examples of biomolecular interactions which have been successfully studied using SPR:

  • Peptide/protein - protein

  • DNA/RNA - protein

  • protein - cell

  • receptor - cell

  • protein - virus/phage

  • carbohydrate - protein

  • carbohydrate - cell

  • liposome - protein

  • artificial materials - biological matter

  • drugs - protein

  • drugs - DNA/RNA.


In addition to biomolecular interaction studies, SPR sensors can be used to quantify adsorption and desorption processes in non-biological systems. One of these less known applications is the quick evaluation of new detergent formulations by real time analysis of dirt desorption from SPR sensor surfaces.


Sensitive optical detection

Our optical biosensors, which use Surface Plasmon Resonance (SPR), measure refractive index changes close to the interface of a sensor chip in real-time. Such changes occur when biomolecules interact with immobilized ligands and replace water from binding sites on the sensor surface. The refractive index differences are detected via angle changes of a reflected laser beam and are directly related to the amount of sensor surface-bound biomolecules. Using this sensitive technique, our SPR sensors are able to detect protein amounts below 10 pg / mm2. For more details on SPR please check the chapter "SPR principle" in our Tech Note section


Liquid handling systems

Liquid handling systems control the hydrodynamic conditions on the sensor surface, which is essential for the performance of a biosensor system. Generally, today’s liquid handling systems can be divided into two groups: flow through systems and cuvette systems. In flow through systems, fresh sample is continually flown over the sensor surface. This method has the advantage of well-defined hydrodynamic conditions during a measurement without rebinding during the dissociation phase. However, the continuous sample consumption limits the maximal association time and, once the sample has been injected, it is difficult to abort the measurement or to inject additional compounds. In cuvette systems, the sample is injected into a well on the sensor surface and is continually mixed during the measurement. This principle is flexible enough to allow multiple sample additions, and it is relatively easy to interrupt the experiment at any time. Moreover, it is possible to measure binding processes with a relatively small sample volume over a very long time or until equilibrium. The latter is especially useful for analytes with a low association constant. For our SPR biosensors flow through and cuvette systems are available. The user thus has the possibility to choose for each experiment the optimal liquid handling system.


Wide variety of sensor surfaces

The sensor surface is the central part of a biosensor. It is here where the biomolecular interaction takes place and where the biochemical binding event is transduced into an opto-electronic signal. Therefore the quality of the sensor surface determines the quality of the interaction plots. As different chemical structures of the immobilized biomolecules require adapted coupling strategies, we offer the market-wide biggest choice of SPR sensordiscs derivatized with various chemical and biochemical functionalities. These functional groups are either linked to planar surfaces or attached to grafted hydrogels which are available in different thicknesses.

xantec | xantec

Weitere Berichte zu: Plasmon SPR Surface

Weitere Nachrichten aus der Kategorie Biowissenschaften Chemie:

nachricht Wie Reize auf dem Weg ins Bewusstsein versickern
22.09.2017 | Rheinische Friedrich-Wilhelms-Universität Bonn

nachricht Lebendiges Gewebe aus dem Drucker
22.09.2017 | Universitätsklinikum Freiburg

Alle Nachrichten aus der Kategorie: Biowissenschaften Chemie >>>

Die aktuellsten Pressemeldungen zum Suchbegriff Innovation >>>

Die letzten 5 Focus-News des innovations-reports im Überblick:

Im Focus: The pyrenoid is a carbon-fixing liquid droplet

Plants and algae use the enzyme Rubisco to fix carbon dioxide, removing it from the atmosphere and converting it into biomass. Algae have figured out a way to increase the efficiency of carbon fixation. They gather most of their Rubisco into a ball-shaped microcompartment called the pyrenoid, which they flood with a high local concentration of carbon dioxide. A team of scientists at Princeton University, the Carnegie Institution for Science, Stanford University and the Max Plank Institute of Biochemistry have unravelled the mysteries of how the pyrenoid is assembled. These insights can help to engineer crops that remove more carbon dioxide from the atmosphere while producing more food.

A warming planet

Im Focus: Hochpräzise Verschaltung in der Hirnrinde

Es ist noch immer weitgehend unbekannt, wie die komplexen neuronalen Netzwerke im Gehirn aufgebaut sind. Insbesondere in der Hirnrinde der Säugetiere, wo Sehen, Denken und Orientierung berechnet werden, sind die Regeln, nach denen die Nervenzellen miteinander verschaltet sind, nur unzureichend erforscht. Wissenschaftler um Moritz Helmstaedter vom Max-Planck-Institut für Hirnforschung in Frankfurt am Main und Helene Schmidt vom Bernstein-Zentrum der Humboldt-Universität in Berlin haben nun in dem Teil der Großhirnrinde, der für die räumliche Orientierung zuständig ist, ein überraschend präzises Verschaltungsmuster der Nervenzellen entdeckt.

Wie die Forscher in Nature berichten (Schmidt et al., 2017. Axonal synapse sorting in medial entorhinal cortex, DOI: 10.1038/nature24005), haben die...

Im Focus: Highly precise wiring in the Cerebral Cortex

Our brains house extremely complex neuronal circuits, whose detailed structures are still largely unknown. This is especially true for the so-called cerebral cortex of mammals, where among other things vision, thoughts or spatial orientation are being computed. Here the rules by which nerve cells are connected to each other are only partly understood. A team of scientists around Moritz Helmstaedter at the Frankfiurt Max Planck Institute for Brain Research and Helene Schmidt (Humboldt University in Berlin) have now discovered a surprisingly precise nerve cell connectivity pattern in the part of the cerebral cortex that is responsible for orienting the individual animal or human in space.

The researchers report online in Nature (Schmidt et al., 2017. Axonal synapse sorting in medial entorhinal cortex, DOI: 10.1038/nature24005) that synapses in...

Im Focus: Tiny lasers from a gallery of whispers

New technique promises tunable laser devices

Whispering gallery mode (WGM) resonators are used to make tiny micro-lasers, sensors, switches, routers and other devices. These tiny structures rely on a...

Im Focus: Wundermaterial Graphen: Gewölbt wie das Polster eines Chesterfield-Sofas

Graphen besitzt extreme Eigenschaften und ist vielseitig verwendbar. Mit einem Trick lassen sich sogar die Spins im Graphen kontrollieren. Dies gelang einem HZB-Team schon vor einiger Zeit: Die Physiker haben dafür eine Lage Graphen auf einem Nickelsubstrat aufgebracht und Goldatome dazwischen eingeschleust. Im Fachblatt 2D Materials zeigen sie nun, warum dies sich derartig stark auf die Spins auswirkt. Graphen kommt so auch als Material für künftige Informationstechnologien infrage, die auf der Verarbeitung von Spins als Informationseinheiten basieren.

Graphen ist wohl die exotischste Form von Kohlenstoff: Alle Atome sind untereinander nur in der Ebene verbunden und bilden ein Netz mit sechseckigen Maschen,...

Alle Focus-News des Innovations-reports >>>

Anzeige

Anzeige

IHR
JOB & KARRIERE
SERVICE
im innovations-report
in Kooperation mit academics
Veranstaltungen

11. BusinessForum21-Kongress „Aktives Schadenmanagement"

22.09.2017 | Veranstaltungen

Internationale Konferenz zum Biomining ab Sonntag in Freiberg

22.09.2017 | Veranstaltungen

Die Erde und ihre Bestandteile im Fokus

21.09.2017 | Veranstaltungen

 
VideoLinks
B2B-VideoLinks
Weitere VideoLinks >>>
Aktuelle Beiträge

11. BusinessForum21-Kongress „Aktives Schadenmanagement"

22.09.2017 | Veranstaltungsnachrichten

DFG bewilligt drei neue Forschergruppen und eine neue Klinische Forschergruppe

22.09.2017 | Förderungen Preise

Lebendiges Gewebe aus dem Drucker

22.09.2017 | Biowissenschaften Chemie